Experiment data/files

The Applied Biosystems™ Analysis Software can import and analyze experiment files (EDS and SDS) that are generated by various Thermo Fisher Scientific real-time qPCR instruments. Every consumable run on a Thermo Fisher Scientific real-time qPCR instrument requires the creation of one or more experiment files that store the associated data. Each experiment file is a virtual representation of a specific consumable (plate, array, or chip) that contains data for all aspects of the qPCR experiment.

Experiment files contain the following information:

  • Target information and arrangement on the plate

  • Sample information and arrangement on the plate

  • Method parameters for the run

File compatibility

The Applied Biosystems™ Software can import data the following experiment file formats generated by Applied Biosystems™ real-time qPCR instruments:

IMPORTANT! The Applied Biosystems™ Analysis Software can import and analyze data from unsupported versions of the instrument software, but we cannot guarantee the performance of the software or provide support for the analyses.

Real-Time PCR System

Supported software version(s)

File extension

Applied Biosystems™ 7900 HT Fast Real-Time PCR System

v2.4 or later

SDS

Applied Biosystems™ 7500 and 7500 Fast Real-Time PCR System

v1.4.1 or later

v2.0.5 or later

EDS

Applied Biosystems™ StepOne™ and StepOnePlus™ Real-Time PCR System

v2.0.1, v2.1, or later

Applied Biosystems™ ViiA™ 7 Real-Time PCR System

v1.1 or later

Applied Biosystems™ QuantStudio™ 12K Flex Real-Time PCR System

v1.1.1 or later

Applied Biosystems™ QuantStudio™ 1 Real-Time PCR System

v1.0 or later

Applied Biosystems™ QuantStudio™ 3 Real-Time PCR System

v1.0 or later

Applied Biosystems™ QuantStudio™ 5 Real-Time PCR System

Applied Biosystems™ QuantStudio™ 6 Flex Real-Time PCR System

v1.0 or later

Applied Biosystems™ QuantStudio™ 6 Pro Real-Time PCR System

Applied Biosystems™ QuantStudio™ 7 Flex Real-Time PCR System

Applied Biosystems™ QuantStudio™ 7 Pro Real-Time PCR System

7500 and 7900HT System standard curve experiment files

By design, the Applied Biosystems™ Analysis Software reads older 7500 and 7900HT System standard curve experiment (SDS) files with the following characteristics as genotyping experiment files. To configure the files to read as standard curve experiments, rename the detectors that are used by the files so that the names do not contain "FAM" or "VIC".

Property

Value

Experiment type

SC (standard curve)

Instrument type

7500 or 7900

Plate setup

Non-empty wells contain exactly two (2) detectors, where:

Detector 1 configuration:

  • Name: FAM

  • Reporter Dye: FAM™ dye

Detector 2 configuration:

  • Name: VIC

  • Reporter Dye: VIC™ dye

Related Topics
Parent topic: Manage experiment data