Multicomponent plot
The Multicomponent Plot is a plot of the complete spectral contribution of each dye for the selected well during the PCR run.
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Toolbar – Contains the following tools for controlling the plot:
– Select individual data points from the plot.
– Allows you to click and manually move the position of the plot.
– Zoom the plot to the selected area.
– Zooms out the plot to show all data points.
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Target/Sample dropdown list – Selects the data (target- or sample-centric) displayed by the plot.
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Normalized fluorescence – Displays the normalized fluorescence for all wells throughout the thermal cycling protocol.
– Saves the plot as an image (PNG or JPG).
– Allows you to adjust the display options for the plot.
When you analyze your own experiment, confirm the following:
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The passive reference dye fluorescence level should remain relatively constant throughout the PCR process.
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The reporter dye fluorescence level should display a flat region corresponding to the baseline, followed by a rapid rise in fluorescence as the amplification proceeds.
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There should not be any spikes, dips, and/or sudden changes in the fluorescent signal.
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There should not be any amplification in negative control wells.