Using the Amplification Plot Histogram
When an Amplification Plot for a specific sample, target, or plate includes data for more than 384 data points, the Applied Biosystems™ Software displays a subset of the data (shown in color) for active viewing against a background of the full dataset (shown in grey). The range of active data in the plot is controlled through a histogram of a numerical attribute associated with the well data, which is located beneath the plot (see below).
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Click and drag the anchor icon (
) to the desired location in the histogram to display the curves from the 384 data points with values nearest to the position of the icon.
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Select the heading of a column in the Well Table that contains numerical content (such as, Amp Score or CT/CRT without Flags) to change the x-axis content of the histogram.
Note: Selecting the heading of a column in the Well Table that contains less than 384 data points hides the histogram. The feature is present only when the plot contains more than 384 amplification curves.