Optimize your sequence to ensure best expression
Use this function if you want to maximize the expression of your sequence and are not concerned about silent mutations (i.e., changes in individual bases that do not change the resulting protein sequence).
- Select your project in the Load tab, then click the Revise tab.
- Click the name of the sequence to display it in the central pane.
- In the Fix or Optimize pane, select the desired target organism from the Organism dropdown list.
- Select an ORF (open reading frame) or coding region from the ORF dropdown list or select Define Manually, then type the ORF.
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Click
to apply the ORF selection.
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(Optional) Click Protect my cloning sites to define which sites to use for cloning, then select an appropriate cloning site present within the sequence, then click Save.
Protect my cloning site is used to prevent:
- (Optional) Click (Protected Regions) Add new to add regions that you do not want to be altered, then click Save.
- (Optional) Click (Motifs to avoid) Add new to add a motif that you do not want to be added to the sequence during optimization, then select the motif from the list, then click Save.
- Click Full Optimization.
- (Optional) When satisfied with your sequences, click Order sequences to go to the Order tab where you can order your sequences.