Preset pipetting functions
Function |
Description |
---|---|
Forward |
The Forward mode (forward pipetting) is recommended for aqueous solutions, such as buffers, diluted acids or alkalis. |
Stepper |
Repeated dispensing of a selected volume is possible with the Stepper mode. The Stepper mode is particularly suitable for microplate applications. |
Reverse |
The Reverse mode (reverse pipetting) is recommended for viscous and volatile liquids. It is also recommended for solutions with low surface tension (e.g., liquids with foaming tendency). In this mode, an excess amount of liquid is aspirated in addition to the set volume. This mode is also useful when performing reagent addition where no purge is desired when dispensing. |
Repetitive |
The Repetitive mode is recommended when the reverse pipetting technique is needed for a few repeated dosings of the same volume. This is particularly useful when working with volumes close to the maximum volume of the pipette. |
Dilute |
The Dilute mode enables the user to dispense two selected volumes with an air gap in-between. This is useful, for instance, when making standard curves. |
Forward + Mix |
The Forward mode (manual pipetting) is recommended for aqueous solutions, such as buffers, diluted acids or alkalis. A mixing step is added after dispensing of the liquid. |
Dilute + Mix |
With the Dilute + Mix mode it is possible to dispense two selected volumes with an air gap in-between, followed by mixing of the liquid. |
Manual |
The Manual mode can be used in different applications. It can be used, for instance, to evaluate the amount of remaining liquid in the reservoir or to dispense small amounts of liquid when slow speeds are needed (e.g., gel electrophoresis). The volume reset function can be utilized, for instance, in titration. |