About the SeqScreener Gene Edit Confirmation App on Thermo Fisher™ Connect

The SeqScreener Gene Edit Confirmation App (SGC) is an application available through Thermo Fisher™ Connect that can be used to determine the spectrum and frequency of targeted mutations generated in a pool of cells by genome editing tools such as CRISPR-Cas9. Use the SGC app to screen and validate gene editing results obtained using Capillary Electrophoresis (CE) sequencing technology.

The SGC app includes the following features:

• An algorithm that accurately reconstructs the spectrum of Indels from the sequence traces and generates a report

• A report output that identifies the detected Indels and their frequencies

• Detection of designed mutations generated by homologous recombination using a donor template

• Compatibility with both knock-in and knock-out experiments

• Batch import capability

• Pool screening and clonal screening analyses